Figure 4

Apoptotic morphological changes of MDA-MB-231 cells following treatment with fractions F1 or F2. Fluorescent micrographs of control (0.5% DMSO) and MDA-MB-231cells treated with 25 μg/ml of F1 or F2 fractions for 48 hours and stained with DAPI (A) or annexin (B) and examined by fluorescence microscopy. Arrows in A indicate apoptotic bodies of nuclear fragmentation. Scale bar is 10 μm.