Alterations in mitochondrial functions following YK treatment of SKOV6 cells. A, Confocal images of JC1 stained cells treated with YK for 24 h. The green fluorescence shows depolarized mitochondria (monomer), whereas red fluorescence shows hyperpolarized (aggregates) mitochondria. B, Cells treated with YK for 24 h showing the release of cytochrome c from mitochondria during apoptosis. Cells were co-transfected with cytochrome c-GFP fusion construct and dsRed mitotracker and visualized by confocal microscopy. Green represents cytochrome c, red mitochondria and orange indicates co-localization of cytochrome c and mitochondria. Results in both panels are represented as mean of three independent experiments ± SD Level of significance; *, p <0.05; **, p <0.001.