Figure 2

Induction of apoptosis by YK in SKOV6 ovarian cells. A, Cells were treated with indicated doses of YK and/or paclitaxel [Lanes: 1-Control, 2-Paclitaxel 10nM, 3-Pac + YK (10nM + 100 μg/ml), 4–7 YK (1, 10, 100, and 200 μg/ml respectively) and 8-50 bp ladder] for 24 h. Genomic DNA was isolated and resolved by agarose gel electrophoresis. B, Cells were treated with different doses of YK (1, 10, 100 μg/ml) was visualized by TUNEL assay. The TUNEL positive cells (stained by FITC) were counted from random fields and are presented in the graph. Nuclei were visualized by DAPI staining (blue). Results are represented as mean of three independent experiments ± SD. Level of significance; *, p <0.001. C, Induction of cell cycle regulators. SKOV6 cells were treated for 24 h with YK (100 μg/ml), Paclitaxel (Pac) (10 μM) or both and the expression of p21, p53, Mdm2, cyclin B1, and PCNA was verified by western blot. GAPDH was used as internal control.