Table 1 Sequences of probe and oligonucleotides used in real-time RT-PCR analysis
Genes | Probe and Primer | Sequence |
|---|---|---|
Leptin | sense | 5′-AACCCTTACTGAACTCAGATTGTTAG-3′ |
antisense | 5′-TAAGTCAGTTTAAATGCTTAGGG-3′ | |
UCP-2 | sense | 5′-TTCAAATGAGATTGTGGGAAAAT-3′ |
antisense | 5′-ACCGATACAGTACAGTACAGTA-3′ | |
Adiponectin | sense | 5′-CCCAAGGGAACTTGTGCAGGTTGGATG-3′ |
antisense | 5′-GTTGGTATCATGGTAGAGAAGAAAGCC-3′ | |
PPAR-γ | FAM | 5′-TCGGAATCAGCTCTGTGGACCTCTCC-3′ |
GAPDH | VIC | 5′-TGCATCCTGCACCACCAACTGCTTAG-3′ |