Effects of ISSE on the recruitment of inflammatory cells in bronchoalveolar lavage fluid (BALF) of mice. Mouse BALF was collected 48 h after final OVA challenge. Cell numbers within at least five squares of a hemocytometer were counted using a light microscope. Dead cells were excluded using trypan blue staining. NC, negative control (PBS only); OVA, OVA-sensitized/challenged mice; Mon, montelukast (30 mg/kg) + OVA-sensitized/challenged mice; ISSE-100, ISEE (100 mg/kg) + OVA-sensitized/challenged mice; ISSE-200, ISEE (200 mg/kg) + OVA-sensitized/challenged mice. Significantly different from NC, #
P < 0.05, ##
P < 0.01; significantly different from OVA, *
P < 0.05, **
P < 0.01.