Danggui extract reversed the Ang II-reduced cardiac survival pathway in H9c2 cardiomyoblast cells. H9c2 cells were treated with Ang II (5×10−7 M) for 1 hr, and then treated with Danggui extract (50, 100 μg/ml) either 1 hr before (pre-treatment) or 1 hr after (post-treatment) Ang II treatment, followed by another 23 hr of culture. Western blotting analysis of (A) Bcl-xL and p-Bad expressions, and (B) p-PI3K, PI3K, p-Akt and Akt. (C) pro-caspase-3 and caspase-3 expression in H9c2 cells treated with inhibitors U0126 (MEK1 and MEK2 inhibitor) and SP600125 (JNK inhibitor). α-tubulin was used as the internal control.