Danggui extract reverses the Ang II-induced instability of the mitochondria membrane potential and cytochrome c expression. (A) Ang II induced instability of the mitochondria membrane potential, as detected by Fluorescein isothiocyanate- (FITC-) JC-1 staining in H9c2 cells. The red image represents that the stable state of Danggui extract could stabilize the mitochondria membrane potential and the green image shows the cells in an unstable state. (B) H9c2 cells were treated with Ang II (5x10−7 M) for 1 hr, and then treated with Danggui extract (50, 100 μg/ml) either 1 hr before (pre-treatment) or 1 hr after (post-treatment) Ang II treatment, followed by another 23 hr of culture. The protein expression of cytochrome c was assessed by Western blotting. α-tubulin was employed as the internal control.