Table 1 Effect of Polygonum minus extract on COX-1 & COX-2, 5-LOX and sPLA2 inhibition
Receptor/enzyme | Source | Substrate | % Inhibition of standard | % Inhibition of Polygonum minus at doses | ||
|---|---|---|---|---|---|---|
Standard | % of inhibition | 30 μg/ml | 100 μg/ml | |||
COX-1 | Ovine platelets | Arachidonic acid | Diclofenac (1 μM) | 100 | 30 | 100 |
COX-2 | Human recombinant | Arachidonic acid | Diclofenac (1 μM) | 100 | 00 | 25 |
5-LOX | Human recombinant Lipooxygenase | Arachidonic acid | NGDA (100 μM) | 100 | 100 | 100 |
sPLA2 | Human recombinant Type V sPLA2 | Diheptanoyl thio-PC | TEPC (20 μM) | 90 | 00 | 00 |