Effect of WEAO on RANKL-induced bone destruction in mice. Mice were orally administrated with vehicle (n = 8 per group) or WEAO (0.25 g/kg, n = 7) twice daily for 5 days, and RANKL (1 mg/kg) was injected intraperitoneally on days 3 and 4. Blood serum and right femur were collected on day 6. (A) Representative micro- CT images of the distal femurs from each group. (B) Quantitative micro- CT analysis of trabecular bone volume/tissue volume (BV/TV), trabecular number (Tb.N), trabecular thickness (Tb.Th), and trabecular separation (Tb.Sp) in the distal femoral metaphysis. (C) Serum TRAP 5b activity. (D) Serum CTX levels. (E) Serum osteocalcin levels. *p < 0.05; **p < 0.01; ***p < 0.001; n.s, not significant.