Figure 2
From: Water extract of the fruits of Alpinia oxyphylla inhibits osteoclast differentiation and bone loss
Effect of WEAO on the gene expression of transcription factors involved in osteoclast differentiation. (A, B) BMMs were cultured with vehicle (distilled water) or WEAO (80 μg/mL) in the presence of M-CSF (60 ng/mL). Total RNA and cell lysates were obtained following stimulation with RANKL (100 ng/mL) for the indicated times. (A) Relative mRNA expression levels of NFATc1, c-Fos, Id2, and MafB were determined by qPCR. **p < 0.01 versus vehicle-treated control. (B) Total cell lysates were subjected to Western blot analysis with antibodies against NFATc1, c-Fos, and p38. p38 was used as a loading control. (C) BMMs transduced with retroviruses expressing either pMX-IRES-GFP (pMX) or pMX-CA-NFATc1-IRES-GFP (pMX-CA-NFATc1) were cultured with vehicle or WEAO (80 μg/mL) in the presence of M-CSF (60 ng/mL) and RANKL (100 ng/ml) for 4 days. The number of osteoclasts (Oc) was counted. **p < 0.01.