Figure 5
From: Anti-osteoclastogenic activity of matairesinol via suppression of p38/ERK-NFATc1 signaling axis
Matairesinol did not affect survival or anti-resorptive activity of mature osteoclasts. (A) Mature osteoclasts were plated on BioCoat slides and treated for 24 h with the indicated concentrations of matairesinol. Cells were fixed, permeabilized, stained with TRAP, and photographed under a light microscope. TRAP-positive multinucleated cells were counted. (B) Attached cells on BioCoat Osteologic MultiTest slides were removed and photographed under a light microscope. Pit areas were quantified using the ImageJ program.