Figure 2

Matairesinol inhibits RANKL-induced expression of NFATc1. (A) BMMs were stimulated with RANKL (10 ng/ml) and M-CSF (30 ng/ml) in the presence or absence of matairesinol (10 μM) for the indicated times. Total RNA was then isolated using TRIzol reagent, and mRNA expression levels were evaluated by RT-PCR. Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) was used as the internal control. (B) Effect of matairesinol on protein expression level of NFATc1 was evaluated by Western blot analysis. Actin was used as the internal control.