Evaluation of in vitro and in vivo anti-inflammatory activity of biologically active phospholipids with anti-neoplastic potential in porcine model

Table 1 Gene specific primers used to assess the anti-inflammatory effect of BAF

Gene	Primer sequence (5′ - 3′)	Gene characteristic/Primer reference
IL-1β/LAF^a	F: GGGACTTGAAGAGAGAAGTGG	Pro-inflammatory/[31]
IL-1β/LAF^a	R: CTTTCCCTTGATCCCTAAGGT	Pro-inflammatory/[31]
TNF-α/TNFSF2^b	F: CCCCCAGAAGGAAGAGTTTC	Pro-inflammatory/[32]
TNF-α/TNFSF2^b	R: CGGGCTTATCTGAGGTTTGA	Pro-inflammatory/[32]
CXCL10/IP10^c	F: CCCACATGTTGAGATCATTGC	Pro-inflammatory/[33]
CXCL10/IP10^c	R: CATCCTTATCAGTAGTGCCG	Pro-inflammatory/[33]
IL-10/B-TCGF^d	F: TGAAGAGTGCCTTTAGCAAGCTC	Anti-inflammatory/[34]
IL-10/B-TCGF^d	R: CTCATCTTCATCGTCATGTAGGC	Anti-inflammatory/[34]
Arg1/Type I arginase	F: CCAGTCCATGGAGGTCTGTC	Anti-inflammatory/[34]
Arg1/Type I arginase	R: GTGTCTTCCCCAGAGATGGA	Anti-inflammatory/[34]
TBP-1	F: AACAGTTCAGTAGTTATGAGCCAGA	Reference gene, RNApolymerase II transcription initiation/[35]
TBP-1	R: AGATGTTCTCAAACGCTTCG
HMBS-2	F: AGGATGGGCAACTCTACCTG	Reference gene, heme biosynthesis/[35]
HMBS-2	R: GATGGTGGCCTGCATAGTCT	Reference gene, heme biosynthesis/[35]
HPRT-1	F: GAGCTACTGtAATGACCAGTCAACG	Reference gene, purine ribonucleoside salvage/[36]
HPRT-1	R: CCAGTGTCAATTATATCtTCAACAATCAA	Reference gene, purine ribonucleoside salvage/[36]

^aLAF = Lymphocyte-activating factor, ^bTNFSF2 = TNF ligand superfamily member 2, ^cIP10 = Interferon gamma-induced protein 10, ^dB-TCGF = B-cell derived T-cell growth factor. F = Forward primer, R = Reverse primer.

ISSN: 2662-7671