Figure 5

Downregulation of caspase-1 activation and PKCϵ phosphorylation in porcine monocyte-derived macrophages by biologically active phospholipids. The activation of caspase-1 and phosphorylation of PKCϵ decreased after 0.3% BAF incubation comparing with control (without biologically active phospholipids) and cells treated with lower concentrations (0.03% and 0.1%) of biologically active phospholipids. A higher anti-inflammatory effect of biologically active phospholipids was observed after 48 h of BAF incubation. β-actin served as loading control.