Figure 2

Scanning electron micrograph of the 48 h biofilm formed in C. albicans 04 on catheter discs in the absence and presence of sub-MICs of oils. (A) Biofilm formed without oils (a. inset) arrow indicates smooth cell membrane of normal cell. (B) Biofilm formed in the presence of C. copticum at 90 μg.mL⁻¹ (b. inset) bursting of cells leading to vesicle formation due to lytic material (ii). (C) Biofilm formed in the presence of T. vulgaris at 90 μg.mL⁻¹ (c. inset) cell membrane shrinkage in sessile cells (iii), empty cells (iv). (D) Biofilm formed in the presence of thymol at 90 μg.mL⁻¹ (d. inset) lysis of membrane and release of cellular material (v).