Figure 4

Measurement of cell detachment and morphological alterations. A: Cell detachment was calculated by flow cytometry after 48 h after exposure to 10 μg/ml of the plant extracts and 0.1% DMSO. (mean ± SD, n = 8, **P < 0.01, *P < 0.05, significantly different compared to control, unpaired t-test). B: Bright field microscopy to monitor morphological alterations after treatment with the four plant extracts JCP1, PS, ZI, JCP2 in a concentration dependent manner on MCF-7 cells. Even the lowest concentration of 1 μg/ml caused a significant detachment of the MCF-7 cells from the plate surface. At a concentration of 10 μg/ml almost 70 - 80% of all cells treated with PS, JCP1 and JCP2 were detached. Detachment correlates with induction of apoptosis which was determined by two-color fluorescent Live/Dead-Staining. Green: viable cells; red: dead cells.