Figure 3

Protein expression analysis of MCF-7 cells by Western blotting after treatment with 10 μg/ml of the four plant extracts in comparison with the control treatment for 48 h. A: Determination of caspase 7, 8, and 9 cleavage. Detected were the pro-caspases as well as the cleaved proteins. B: Expression of total focal adhesion kinase (FAK) protein and phosphorylated FAK protein at residue Tyr397. C: Protein content of kinases: total Akt, phosphorylated Akt at residue S473 (pAkt), total MAPK p44/42, phosphorylated MAPK p44/42 at residues T202/204 (P-p44/42) and β-Actin which did not function as housekeeping protein. D: Stain-free image of separated proteins after SDS-PAGE to ensure equal protein amounts on each polyacrylamide gel used.