Figure 2

Isolated epidermal cells that were pretreated with TSA for 2 weeks showed enhanced resistance to CoCl ₂ , which was used to mimic hypoxia in vitro. After exposure to CoCl₂ for 72 h, nuclear DAPI staining revealed larger number of TSA-pretreated cells than control cells (A). A CCK-8 assay demonstrated that TSA-pretreated cells showed more proliferation than control cells after exposure to CgCl₂ for 96 h (B). A Trypan Blue assay showed that the survival rate of TSA-pretreated cells was higher than that of control cells after treatment with CoCl₂ (C).