Figure 2
From: Antimutagenic and anticancer activity of Darjeeling tea in multiple test systems
Flow cytometric detection of apoptosis induction by three different doses of DTE at 48 h of treatment. U937 cells (1 × 106 per treatment) were incubated with DTE at 0 μg/ml (A), 25 μg/ml (B), 50 μg/ml (C) and 100 μg/ml (D) doses for 48 h. DTE induced apoptosis in U937 cells was determined by the flow cytometry using double staining method. Vehicle (0.05% DMSO only) treated control (A) and DTE treated cells (B, C, D) were labeled with PI and AnnexinV tagged-Alexa Fluor and then fixed and analyzed by flow cytometer. Dual parameter dot plot of Alexa fluor-fluorescence (X-axis) versus PI fluorescence (Y-axis) has been shown in logarithmic fluorescence intensity. Quadrants: lower left, live cells (- Alexa Fluor), lower right, apoptotic cells (+Alexa Fluor), upper right, necrotic cells or late phase of apoptotic cells (+PI, + Alexa Fluor). Numbers represented in each block here is the% of cells of lower right quadrant only.