Figure 4

Temporal evolutions of the antidepressant-like effects caused by HCE75, the standardized flavonoid mixture containing equivalent doses of rutin, hesperidin, quercetin, and quercitrin and the fixed-ratio combination of rutin and hesperidin (75:21.5, w/w) on mice in TST. The mice were tested 0, 0.5, 1, 2, 3, 4, and 8 h after administering (p.o.) with the vehicle (physiological saline with 10% Tween 80), HCE75 (400 mg/kg), standardized flavonoid mixture (8 mg/kg), and rutin/hesperidin fixed-ratio combination (8 mg/kg). Data are expressed as mean ± S.E.M. (n = 10). Data were analyzed using one-way ANOVA for multiple comparisons, followed by post hoc Student–Newman–Keuls test. *p < 0.05, **p < 0.01, and ***p < 0.001 compared with the control group (tested 1 h after vehicle treatment).