Effects of HCE on immobility time in TST (A) and line crossings in OFT (B). The apparent correlations between (C) chemical and (D) flavonoid constituent dosages and the immobility time in HCE in the TST. The results are expressed as% immobility time relative to the control group (vehicle) for the antidepressant-like effects. The mice were tested 60 min after they were administered (p.o.) with the vehicle (physiological saline with 2% Tween 80), fluoxetine (20 mg/kg), and HCE (400 mg/kg). Data are expressed as mean ± S.E.M. (n = 10). Data were analyzed using one-way ANOVA for multiple comparisons, followed by post hoc Student–Newman–Keuls test. *p < 0.05, **p < 0.01, and ***p < 0.001 compared with the control group (vehicle); and #p < 0.05, ##p < 0.01, and ###p < 0.001 compared with the positive reference group (fluoxetine). Linear regression was performed along with the ordinary least squares estimation.