Figure 5
Effect of CA on expression of cell death mediator proteins. Y79 cells treated with CA (50 μM) for different time periods (0, 3, 6, 12, 24 and 48 h). Equal amounts of total protein (BAX, Bcl2 and β-actin) and cytosolic protein (Cytochrome c) (100 μg) was analysed by SDS- PAGE 7–12%, and after electrophoresis, proteins on the gel were transferred on to nitrocellulose membrane and probed with protein-specific antibody. Lane 1, 0 h; lane 2, 3 h; lane 3, 6 h; lane 4, 12 h; lane 5, 24 h; lane 6, 48 h. (A) BAX; (B) Bcl2; (C) Cytochrome c (D) β-actin. Bar graphs represents mean relative expression of protein normalised against β-actin.