Skip to main content

Table 1 Experimental design and observed experimental data

From: Optimization of ultrasound-assisted extraction of flavonoid compounds and their pharmaceutical activity from curry leaf (Murraya koenigii L.) using response surface methodology

Treatment Temperature °C Methanol concentration % Ultrasunic power (W) Catechin Predicted Myricetin Predicted Quercetin Predicted Antioxidant activity Predicted
1 40 40 80 0.265 ± 0.011 0.267 0.309 ± 0.025 0.311 0.177 ± 0.010 0.179 67.0 ± 2.20 64.6
2(C) 60 60 115 0.426 ± 0.035 0.453 0.470 ± 0.018 0.497 0.338 ± 0.018 0.365 80.0 ± 7.05 79.5
3 40 40 150 0.274 ± 0.014 0.286 0.318 ± 0.021 0.330 0.186 ± 0.008 0.198 66.2 ± 4.72 65.4
4 80 80 80 0.191 ± 0.002 0.233 0.235 ± 0.017 0.277 0.103 ± 0.011 0.145 52.0 ± 7.76 55.2
5 (C) 60 60 115 0.396 ± 0.005 0.453 0.440 ± 0.016 0.497 0.308 ± 0.017 0.365 79.5 ± 2.30 79.5
6 (C) 60 60 115 0.436 ± 0.017 0.453 0.480 ± 0.007 0.497 0.348 ± 0.019 0.365 77.0 ± 3.82 79.5
7 80 40 150 0.234 ± 0.008 0.247 0.278 ± 0.014 0.291 0.146 ± 0.009 0.159 55.4 ± 1.81 57.19
8 80 40 80 0.222 ± 0.012 0.247 0.266 ± 0.011 0.291 0.134 ± 0.014 0.159 55.0 ± 2.30 55.4
9 40 80 150 0.347 ± 0.011 0.376 0.391 ± 0.014 0.420 0.259 ± 0.012 0.288 71.0 ± 3.01 73.14
10 40 80 80 0.295 ± 0.007 0.335 0.339 ± 0.017 0.380 0.207 ± 0.011 0.247 69.0 ± 5.56 69.75
11 80 80 150 0.204 ± 0.019 0.255 0.244 ± 0.006 0.296 0.116 ± 0.007 0.167 54.7 ± 4.12 59.59
12(C) 60 60 115 0.474 ± 0.010 0.453 0.518 ± 0.026 0.497 0.386 ± 0.016 0.365 78.0 ± 2.69 79.5
13 86.32 60 115 0.188 ± 0.015 0.213 0.232 ± 0.019 0.247 0.100 ± 0.011 0.105 51.0 ± 1.88 53.5
14(C) 60 60 115 0.466 ± 0.008 0.453 0.510 ± 0.013 0.497 0.378 ± 0.017 0.365 80.4 ± 3.27 79.5
15 60 60 161.06 0.480 ± 0.017 0.454 0.524 ± 0.019 0.498 0.392 ± 0.020 0.366 83.0 ± 4.61 79.7
16 60 86.32 115 0.536 ± 0.014 0.464 0.580 ± 0.018 0.507 0.448 ± 0.015 0.376 87.0 ± 3.55 84.8
17 60 60 68.93 0.456 ± 0.018 0.434 0.500 ± 0.015 0.478 0.368 ± 0.013 0.346 75.0 ± 2.70 77.1
18 60 33.67 115 0.403 ± 0.018 0.426 0.447 ± 0.008 0.470 0.315 ± 0.010 0.338 73.0 ± 3.29 75.2
19 33.67 60 115 0.244 ± 0.014 0.285 0.288 ± 0.017 0.317 0.156 ± 0.016 0.188 58.0 ± 1.77 60.9
20(C) 60 60 115 0.460 ± 0.010 0.453 0.504 ± 0.017 0.497 0.372 ± 0.019 0.365 79.5 ± 2.11 79.5
  1. Catechin, myricetin and quercetin identified by UHPLC and antioxidant activity was measured by DPPH assay.
  2. (C): central points; Catechin, Myricetin and Quercetin content are in mg g-1 DW. DPPH activity is in %.