Optimization of ultrasound-assisted extraction of flavonoid compounds and their pharmaceutical activity from curry leaf (Murraya koenigii L.) using response surface methodology

Ghasemzadeh, Ali; Jaafar, Hawa ZE; Karimi, Ehsan; Rahmat, Asmah

doi:10.1186/1472-6882-14-318

BMC Complementary Medicine and Therapies

Table 1 Experimental design and observed experimental data

From: Optimization of ultrasound-assisted extraction of flavonoid compounds and their pharmaceutical activity from curry leaf (Murraya koenigii L.) using response surface methodology

Treatment	Temperature °C	Methanol concentration %	Ultrasunic power (W)	Catechin	Predicted	Myricetin	Predicted	Quercetin	Predicted	Antioxidant activity	Predicted
1	40	40	80	0.265 ± 0.011	0.267	0.309 ± 0.025	0.311	0.177 ± 0.010	0.179	67.0 ± 2.20	64.6
2(C)	60	60	115	0.426 ± 0.035	0.453	0.470 ± 0.018	0.497	0.338 ± 0.018	0.365	80.0 ± 7.05	79.5
3	40	40	150	0.274 ± 0.014	0.286	0.318 ± 0.021	0.330	0.186 ± 0.008	0.198	66.2 ± 4.72	65.4
4	80	80	80	0.191 ± 0.002	0.233	0.235 ± 0.017	0.277	0.103 ± 0.011	0.145	52.0 ± 7.76	55.2
5 (C)	60	60	115	0.396 ± 0.005	0.453	0.440 ± 0.016	0.497	0.308 ± 0.017	0.365	79.5 ± 2.30	79.5
6 (C)	60	60	115	0.436 ± 0.017	0.453	0.480 ± 0.007	0.497	0.348 ± 0.019	0.365	77.0 ± 3.82	79.5
7	80	40	150	0.234 ± 0.008	0.247	0.278 ± 0.014	0.291	0.146 ± 0.009	0.159	55.4 ± 1.81	57.19
8	80	40	80	0.222 ± 0.012	0.247	0.266 ± 0.011	0.291	0.134 ± 0.014	0.159	55.0 ± 2.30	55.4
9	40	80	150	0.347 ± 0.011	0.376	0.391 ± 0.014	0.420	0.259 ± 0.012	0.288	71.0 ± 3.01	73.14
10	40	80	80	0.295 ± 0.007	0.335	0.339 ± 0.017	0.380	0.207 ± 0.011	0.247	69.0 ± 5.56	69.75
11	80	80	150	0.204 ± 0.019	0.255	0.244 ± 0.006	0.296	0.116 ± 0.007	0.167	54.7 ± 4.12	59.59
12(C)	60	60	115	0.474 ± 0.010	0.453	0.518 ± 0.026	0.497	0.386 ± 0.016	0.365	78.0 ± 2.69	79.5
13	86.32	60	115	0.188 ± 0.015	0.213	0.232 ± 0.019	0.247	0.100 ± 0.011	0.105	51.0 ± 1.88	53.5
14(C)	60	60	115	0.466 ± 0.008	0.453	0.510 ± 0.013	0.497	0.378 ± 0.017	0.365	80.4 ± 3.27	79.5
15	60	60	161.06	0.480 ± 0.017	0.454	0.524 ± 0.019	0.498	0.392 ± 0.020	0.366	83.0 ± 4.61	79.7
16	60	86.32	115	0.536 ± 0.014	0.464	0.580 ± 0.018	0.507	0.448 ± 0.015	0.376	87.0 ± 3.55	84.8
17	60	60	68.93	0.456 ± 0.018	0.434	0.500 ± 0.015	0.478	0.368 ± 0.013	0.346	75.0 ± 2.70	77.1
18	60	33.67	115	0.403 ± 0.018	0.426	0.447 ± 0.008	0.470	0.315 ± 0.010	0.338	73.0 ± 3.29	75.2
19	33.67	60	115	0.244 ± 0.014	0.285	0.288 ± 0.017	0.317	0.156 ± 0.016	0.188	58.0 ± 1.77	60.9
20(C)	60	60	115	0.460 ± 0.010	0.453	0.504 ± 0.017	0.497	0.372 ± 0.019	0.365	79.5 ± 2.11	79.5

Catechin, myricetin and quercetin identified by UHPLC and antioxidant activity was measured by DPPH assay.
(C): central points; Catechin, Myricetin and Quercetin content are in mg g^-1 DW. DPPH activity is in %.

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ISSN: 2662-7671

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