Effect of AJN pre-treatment on DAT by immunohistochemical staining and autoradiography. A: Representative staining pictures and effect of AJN on DAT by immunohistochemical staining. B: Representative autoradiograms and effect of AJN on DAT by autoradiography with [125I]-β-CIT. Results of the two methods are accordant with each other. AJN pre-treatment completely blocks morphine-induced DAT expression decrease, and AJN post-treatment effectively restores DAT expression. NS: control group; Mor: morphine group. Data are expressed as the means ± SD (*p < 0.05, **p < 0.01, vs. morphine group; n = 5; Student’s t-test).