Table 2 Sequences of the primers in reverse transcription and quantitative real-time PCR
From: Up-regulation on cytochromes P450 in rat mediated by total alkaloid extract from Corydalis yanhusuo
Gene name | Sequence | Product size (bp) |
|---|---|---|
β-actin |  |  |
 Forward | ACCCCAAAGCCAACAGAGAG | 102 |
 Reverse | AGGCATACAGGGACAGCACA |  |
CYP1A2 | Â | Â |
 Forward | CATAGCCTCAGACCCCACAT | 165 |
 Reverse | ATGGCTCCGATGACATTAGC |  |
CYP2C11 | Â | Â |
 Forward | AGGGCCTTGGAGTCATTTTT | 163 |
 Reverse | GCACCTTTGCTCTTCCTCAG |  |
CYP2D1 | Â | Â |
 Forward | GCTGACAAGGTCTTCCAAGG | 188 |
 Reverse | ACCACCATGCGTAGGTTCTC |  |
CYYP2E1 | Â | Â |
 Forward | ATGTCATCCCCAAGGGTACA | 184 |
 Reverse | AGGCCTTCTCCAACACACAC |  |
CYP3A1 | Â | Â |
 Forward | TATGGGGAAAGCCATCTCTG | 164 |
 Reverse | CAGGTTTGCCTTTCTCTTGC |  |