Table 1 MRM transitions and collosion energies for the detection of CYPs probe substrate metabolites
From: Up-regulation on cytochromes P450 in rat mediated by total alkaloid extract from Corydalis yanhusuo
Substrate metabolite | Molecular mass (MW) | Precursor (m/z) | Product (m/z) | Polarity | Collision energy (eV) |
|---|---|---|---|---|---|
Paracetamol (PAR) | 151 | 152 | 110 | ESI+ | 23 |
Dextrorphan (DEXP) | 257 | 258 | 199 | ESI+ | 38 |
1-Hydroxymidazolam (OHMDZ) | 341 | 342 | 203 | ESI+ | 40 |
4-Hydroxytolbutamide (OHTOL) | 286 | 287 | 171 | ESI+ | 59 |
6-hydroxychlorzoxazone (OHCHL) | 185 | 184 | 120 | ESI- | -26 |
Kamaxiping (internal standard) | 236 | 237. | 194 | ESI+ | 40 |