Figure 6
Effect of AMEAE on cell membrane permeability, MMP and cytochrome c release. (A) Representative images of A549 cells treated with medium alone and 10 μg/mL of AMEAE, and stained with MMP, cell membrane permeability, Hoechst 33342 and cytochrome c dyes. AMEAE caused a marked elevation in cytochrome c leakage and cell membrane permeability, and a noteworthy decrease in MMP. Representative bar charts indicating dose-dependent increased (B) cell permeability and reduced (C) MMP in treated A549 cells at 5 to 40 μg/mL concentrations. Meanwhile, (D) cytochrome c was significantly released at 10 to 40 μg/mL concentrations. The data represent the means ± SEM of three independent experiments. *p < 0.05 compared with the untreated group.