Figure 3

Time-dependent induction of early and late apoptosis by AMEAE indicated the percentage of early apoptotic, late apoptotic and necrotic cells after (B) 24, (C) 48 and (D) 72 h of treatment. (A) shows the cells treated with vehicle DMSO. The early and late apoptotic events are depicted in the lower right and upper left quadrant of each panel. (E) Representative bar chart demonstrated significant elevation in a number of early and late apoptotic cells after 48 h of treatment. The data represent the means ± SEM of three independent experiments. *p < 0.05 compared with the untreated group.