Table 1 The genes, primer sequence for sense (upper row) and anti-sense (lower row) strands references and GenBank accession numbers utilized for real-time PCR are shown
Gene | Primer sequence | Reference | GenBank accession No |
|---|---|---|---|
ALPase | 5′-TGACCTTCTCTCCTCCATCC-35′ | [26] | NM_007431 |
5′-CTTCCTGGGAGTCTCATCCT-3′ | |||
Osteocalcin | 5′-TGCTTGTGACGAGCTATCAG-3′ | [26] | NM_007541 |
5′-GAGGACAGGGAGGATCAAGT-3′ | |||
Col II α1 | 5′-ACTGGTAAGTGGGGCAAGAC-3′ | [26] | NM_031163 |
5′-CCACACCAAATTCCTGTTCA-3′ | |||
Aggrecan | 5′-AGGACCTGGTAGTGCGAGTG-3′ | [27] | NM_007424 |
5′-GCGTGTGGCGAAGAA-3′ | |||
β-actin | 5′-AGATGTGGATCAGCAAGCAG-3′ | [26] | NM_007393 |
5′-GCGCAAGTTAGGTTTTGTC-3′ |