Figure 9
Histochemical analysis of primary MBM cells for differentiation induced by herbal extracts. MBM cells were seeded at a density of 1.5×104 cells /well in 6-well tissue culture plates, and cultured with (Dif) or without (-) commercially available Osteoblast-Inducer Reagent, in the absence (Control) or presence of 1 and 10 μg/ml C. atratum, M. azedarach, and C. turtschaninovii extracts. After 7 d, the cells were subjected to ALPase activity (ALP) or TRAP staining. A, The cells were examined by a microscope, and photographed. Bar, 500 μm. B, The number of positive cells was counted by a microscope, and means ± standard deviation of 3 individual fields were shown. *, p < 0.05 vs each control.