Figure 4
Modulation of apoptosis-related proteins by stimulation with herbal extracts. RAW264.7 cells were allowed to differentiate into OCs. a. The cells were seeded at a density of 1×105 cells /well in 6-well culture plates, and allowed to differentiate into osteoclasts by the addition of RANKL. Thereafter, the cells were cultured in the absence (Cont) or presence of 10 μM AD, or 1, 10 and 100 μg/ml C. atratum (Atratum) M. azedarach (Azedarach) and C. turtschaninovii (Turtschan) extracts. After 3 days, total cellular protein was collected, and 20 μg of the aliquot was subjected to Western Blotting analysis for Bax, Bad, Bak, Bid Bcl-2, Bcl-XL, p53 and GAPDH, sequentially.