Figure 5

Effect of EA on NK-1 expression in the ipsilateral spinal dorsal horn at 49 h after CFA injection. (A). The expression of NK-1 mRNA (relative to GAPDH) in L4-6 ipsilateral spinal dorsal horn measured by real-time PCR. **P < 0.01 versus control group. ^▲▲ P < 0.01 versus model group. ## P < 0.05 versus sham EA group. Results are mean ± SEM; n = 6. (B). The expressions of NK-1 protein in the L4-6 ipsilateral spinal cord dorsal horn by western blotting analysis. **P < 0.01 versus control group. ^▲ P < 0.05 versus model group. Results are mea n ± SEM; n = 6. C. NK-1 protein in normalized against β-actin.