Figure 4

Histopathological and immunohistochemical observation of rat liver sections Vacuolar degeneration (dotted arrows) and hepatocellular necrosis (complete arrows) are displayed). (A): Control group; (B): group treated 12 mg/kg cisplatin; (C): group pretreated with thymoquinone then cisplatin as discussed in methods. Immunohistochemical staining of NF-κB in rat from: (D, 200) group 1, showing no expression of NF-κB; (E, 200) group 2 showing a significant increase in NF-κB immunoreactivity in the cytoplasm of hepatocytes; and (F, 200) group 3 demonstrating a significant reduction in NF-κB immunostaining.