The natural triterpene 3β,6β,16β-trihydroxy-lup-20(29)-ene obtained from the flowers of Combretum leprosum induces apoptosis in MCF-7 breast cancer cells

Table 4 Induction of reversion of point mutation for his1-798 , ochre allele lys1-1 , and frameshift mutation ( hom3-10 ) in haploid strain XV185-14c of S. cerevisiae after treatment of TTHL isolated product from ethanolic extract of C. leprosum

Agent	Treatment (μg/mL)	Survival (%)	LYS1/10⁸survivors^b	HIS1/10⁷survivors^a	HOM3/10⁸survivors^a
STAT cells treated in PBS
NC^d	0	100.00	4.00 ± 2.83^c	10.50 ± 0.71^c	4.0 ± 1.41^c
4-NQO^e	1.0 μg/mL	39.97***	20.85 ± 2.48***	49.50 ± 9.19***	13.50 ± 4.95**
TTHL	10 μg/mL	84.90	4.78 ± 0.63	10.77 ± 4.40	6.11 ± 1.58
	50 μg/mL	72.83	5.68 ± 2.22	13.33 ± 1.10	8.80 ± 0.14
	100 μg/mL	65.00	9.72 ± 0.55	20.83 ± 1.80	8.49 ± 2.12
	500 μg/mL	69.00	14.40 ± 3.40**	30.10 ± 0.21**	11.15 ± 0.92*

^aLocus-specific revertants; ^bLocus non-specific revertants; ^cMean and standard deviation per three independent experiments; ^dNegative control (solvent); ^ePositive control; *Data significant in relation to negative control group (solvent) at *P < 0.05; **P < 0.01; ***P < 0.001/ One-way ANOVA-Tukey’s Multiple Comparison Test.

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