ZJW inhibits P-gp expression and the effect of the PI3K/Akt pathway. (A) Western blotting assay was carried out to detect the level of P-gp, LRP, and MRP-2 in HCT116/L-OHP cells treated with LY294002 (20 μM, 2 h), ZJW (50 μg/mL, 48 h), and a combination of ZJW (50 μg/mL, 48 h) and IGF-1 (100 ng/mL, 48 h). GAPDH was used to ensure equal loading of proteins in each lane. (B) Blots were photographed and quantitated; the data are from three independent experiments. (C) Real-time PCR was performed to detect ABCB1 mRNA in vivo. Quantification of ABCB1 mRNA was performed by assigning a value of 1 to the control group treatment with ZJW (50 μg/mL). Statistical difference was analyzed by student’s t-test, **P < 0.01 vs. control group; #
P < 0.05 vs. ZJW (50 μg/mL) group. This is a representative result of three experiments with similar results.