Figure 2
ZJW reversed constitutive as well as PI3K/Akt pathway-induced MDR phenotype. (A) Anti-proliferative IC50 values of L-OHP on HCT116/L-OHP cell treated with LY294002 (20 μM, 2 h), ZJW (50 μg/mL, 48 h), and a combination of ZJW (50 μg/mL, 48 h) and IGF-1 (100 ng/mL, 48 h), were analyzed by CCK-8. Data are presented as mean ± SD of triplicate experiments. **P < 0.01 vs. control, # P < 0.05 vs. ZJW group. (B) Flow cytometry analysis of apoptosis with Annexin V-FITC/PI binding to HCT116/L-OHP cells treated with LY294002 (20 μM, 2 h), ZJW (50 μg/mL, 48 h), and a combination of ZJW (50 μg/mL, 48 h) and IGF-1 (100 ng/mL, 48 h).