Figure 3

EOPK suppresses AKT and ERK phosphorylation and β-catenin expression via PAK1 inhibition in HCT116 colorectal cancer cells. (A) HCT116 cells were treated with various concentrations of EOPK (25, 50, 100 μg/ml). (B) HCT116 cells were transfected with PAK1-siRNA or a control siRNA for 48 h and incubated in the presence or absence of EOPK (100 μg/ml) for 24 h. For both experiments, cell lysates were prepared and subjected to Western blotting to determine the expression of PAK1, p-PAK1, AKT, p-AKT, ERK, p-ERK, β-catenin and β-actin. Band density of PAK1, p-PAK1, AKT, p-AKT, ERK, p-ERK, and β-catenin was quantified using Gelpro analyzer (Media Cybernetics, Bethesda, MD, USA).