Figure 2

EOPK inhibits cell proliferation and migration in HCT116 colorectal cancer cells. (A) Cells were treated with EOPK (100 μg/ml) for 24 h. Cell cycle distribution was analyzed by flow cytometry. Bar graphs represent the percentage of sub-G1, G1, S, G2-M phase cells. Data represent mean ± SD of three independent experiments. *** p < 0.001 compared with untreated control. (B) HCT116 cells were treated with various concentrations of EOPK (25, 50, 100 μg/ml) and maintained for 5 days. The cells were resolved in 70% ethanol after washing with distilled water, and crystal violet absorbance was read using a microplate reader. Data represent mean ± SD of three independent experiments. * p < 0.05, ** p < 0.01 and *** p < 0.001 compared with control. (C) HCT116 cells were treated with EOPK for 24 h or 48 h and cell proliferation was measured by using a BrdU proliferation ELISA kit (Roche, Swiss). (D) Cells were treated with EOPK (25, 50, 100 μg/ml) for 24 h, and cell migration was assayed by wound healing assay. The number of cells migrating into the scratched area was photographed (×100) and calculated as a percentage of migration. Data are shown as the mean ± SD of three independent experiments. *** p < 0.001 compared with untreated control.