Figure 1

PAK1 mediates MEK/ERK, PI3K/AKT, and Wnt/β-catenin in colon cancer cells. (A) Basal levels of PAK1 expression in colorectal cancer cell lines (human and murine) and normal colon cells extracted from mouse tissue were determined by Western blotting. (B) HCT116 cells were transfected with PAK1-siRNA1, PAK1-siRNA2, or control siRNA for 48 h. Cell lysates were prepared and subjected to Western blotting to determine the expression of PAK1, p-PAK1, AKT, p-AKT, ERK, p-ERK, β-catenin and β-actin. (C) Various human colorectal cancer cells were treated with EOPK (100 μg/ml) for 24 h. (D) Cytotoxicity of EOPK was analyzed by MTT assay in NIH-3 T3 cells as a normal cell control.