Effects of fractioned extracts on TNF-α and IL-1 mRNA expression. Quantitative real-time PCR evaluation for the effects of crude extracts of BQZ decoction on mRNA expression of TNF-α (A) and IL-1 (B). M1-polarized RAW264.7 was stimulated with the different crude extracts (50 μg/ml respectively) for 24 h. The M1-polarized RAW2647 without extracts stimulation were used as control and RAW264.7 without treatment were employed as the additional controls. Total RNA was extracted and subjected to real-time PCR experiments. Data are expressed as mean ± SD of three independent experiments done in triplicate. *p < 0.01 and **P < 0.01 versus the additional controls, while #p < 0.05 and ##p < 0.01 versus control.