Figure 3

EcEE exposure induces mitotic disruption. (A) DAPI stained abnormal mitotic cells after EcEE exposure showing (i) defective chromosome congression, (ii) tripolar and (iii) tetrapolar metaphases and (iv) tripolar anaphase with chromosome bridges, bar = 5 μm. (B) Percentage of abnormal mitosis after 24 h and 48 h exposure to distinct concentrations of EcEE; the total number of mitotic cells scored and utilized to calculate the percentage of abnormal mitosis is shown in brackets. (C) AURKA differential transcription after exposure to 48 h of EcEE. Results are shown as the mean log2 fold change (2^-ΔΔCt) ± standard deviation in relation control, *p < 0.0001.