Figure 2

Nuclear organization is disrupted after 48 h of exposure to EcEE 25 μg/ml. (A) DAPI stained HT29 interphase cells. Nuclear anomalies, namely pyknosis (arrow), micronuclei (open arrow head) and karyorrhexis (arrow head) are detectable only for 25 μg/ml EcEE. The lack of effects induced by EcEE lower concentrations is exemplified by 5 μg/ml EcEE. (B) TUNEL positive nuclei (arrow) with sparse labeling are detectable for 25 μg/ml EcEE. (C) bcl-xl differential expression after 48 h exposure to distinct EcEE concentrations. Results in are shown as the mean log2 fold change (2^-ΔΔCt) ± standard deviation in relation control, **p < 0.0001 and *p < 0.01. (D) DAPI staining (blue) and α-tubulin immunodetection (red in merged images at the bottom) of interphase cells after 48 h in control or 25 μg/ml EcEE. (E) Immunodetection of H3K9ac after 48 h in control or EcEE 25 μg/ml supplemented medium. Within each experiment all images have identical magnification, bar = 5 μm.