Figure 1
HPLC fingerprint of W-LHIT. HPLC conditions: column, Agilent Zorbax SB-C18 column (150 × 4.6 mm i.d.; 5 μm particle size); flow rate, 1 mL/min; column temperature, 27°C; mobile phase A, 0.1% formic acid, mobile phase B, acetonitrile. Data were processed using Waters Empower software. Twenty-one major peaks were present in the HPLC fingerprint. Twelve compounds were characterized by LC-MS.