Figure 3

pJNK and PUMA expression level. Total cell lysates were prepared from control cells, cells treated with FFAs 1 mM for 24 h, and cells pretreated with FFAs 1 mM for 24 h and then cultured with AC extract for 24 h respectively. (A) The overexpressed level of pJNK and PUMA were down-regulated after treatment of AC extract for 24 h. (B) PUMA expression level normalized with β-actin was measured by densitometry analysis and represented as bar charts. β-actin was used as an internal control. Statistical significance was determined by one-way ANOVA and the values are mean ± SEM. ###, p < 0.001, control versus FFAs-treated group (lipoapoptosis-induced group) and ***, p < 0.001, FFAs-treated group versus AC extract treated group.