Oil Red O staining for HepG2 cells and quantitative analysis of lipid. FFAs 1 mM for 24 h induced lipid accumulation in HepG2 cells. Visual observation of lipid content was captured by microscope (×400): the control cells treated with only 1% BSA (A), cells treated with FFAs 1 mM for 24 h (B), and cells pretreated with FFAs 1 mM for 24 h and then cultured with AC extract for 24 h (C). The quantitative analysis of cellular steatosis (D) was measured through deposited Oil Red O in the cells. Statistical significance was determined by one-way ANOVA and the values are mean ± SEM. ###, p < 0.001, control versus FFAs-treated group (lipoapoptosis-induced group) and ***, p < 0.001, FFAs-treated group (lipoapoptosis-induced group) versus AC extract treated group.