Figure 7From: Synergistic anticancer effects of a bioactive subfraction of Strobilanthes crispus and tamoxifen on MCF-7 and MDA-MB-231 human breast cancer cell lines Activation of caspase 8 in MCF-7 and MDA-MB-231 cells by SCS alone and in combination with tamoxifen. Cells were stained with FLICA™ FAM-VAD-FMK for detection of active caspase-8 and labeled with PI and Hoescht stain following treatment with SCS (8.5 and 10.0 μg/ml for MCF-7 and MDA-MB-231 cells, respectively), tamoxifen (5.0 μM) or their combination for 24 h. Caspase-FLICA stains active caspases green; PI stains dead or dying cells red; Hoescht stains the nuclei of all cells blue.Back to article page