Depolarization of the mitochondrial membrane of MCF-7 cells by SCS alone and in combination with tamoxifen. MCF-7 cells treated with 8.5 μg/ml SCS and 2.5 (Tam2.5) or 5.0 μM tamoxifen (Tam5) alone and in combination for 24 and 48 h were incubated with the JC-1 dye and analysed by flow cytometry. (A) Representative dot plots: J-aggregated red fluorescence (polarized cells); JC-1 monomer green fluorescence (depolarized cells). (B) Each bar represents mean ± SD of three independent experiments. *p < 0.05, **p < 0.01, ***p < 0.001 significantly different from control; cp < 0.001 SCS + Tam vs Tam alone (one-way ANOVA and post hoc Tukey multiple comparison test).