Promotion of tamoxifen-induced apoptosis of MDA-MB-231 cells by SCS. Cells were treated with 10.0 μg/ml SCS and 2.5 (Tam2.5) or 5.0 μM tamoxifen (Tam5) alone and in combination for 24 and 48 h. Harvested cells were incubated with Annexin-V antibody and PI dye and analysed by flow cytometry. (A) Quadrant location for the representative dot plots: lower left – negative immunofluorescence (living cells); lower right – annexin V positive (early apoptosis); upper left – PI positive (necrosis), upper right – annexin V and PI positive (late apoptosis). (B) Each bar represents mean ± SD of three independent experiments. *p < 0.05, **p < 0.01, ***p < 0.001 significantly different from control; cp < 0.001 SCS + Tam vs Tam alone (one-way ANOVA and post hoc Tukey multiple comparison test).