Expression of LC β-galactosidase in different experimental groups. Upper panel: Illumination of expression of LC β-galactosidase in different experimental groups. The cytoplasm of positive cells was blue, the normal control group (A) had some blue-stained cells, but there were significantly more blue-stained cells in the aging group (B). The β-galactosidase-positive rate in the aging group was significantly higher than that in the Shouwu pill group (C) and Heshouwuyin group (D). Scale bar, 100 μm. Lower panel: Relative expression of LC β-galactosidase in different experimental groups. NCG: normal control group; SWYC: Heshouwuyin control group; AG: aging group; SWPG: Shouwu pill group; SWYG: Heshouwuyin group. Each histogram represents the mean ± SD of the values for 5 rats. Columns with different letters represent statistically different values, while same letters indicates no significant difference.