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Table 3 HPLC conditions used for the analysis of OR

From: Oryeongsan inhibits LPS-induced production of inflammatory mediators via blockade of the NF-kappaB, MAPK pathways and leads to HO-1 induction in macrophage cells

Item Condition
Mobile phase Time (min) Water (containing 0.1% TFA) Acetonitrile
  0 90 10
  5 90 10
  40 10 90
  40 0 100
  60 0 100
Flow rate 1.0 mL/min
Inject volume 20 μL
Column OptimaPak C18 (4.6 × 250 mm, 5 μm, RS tech Co., Daejeon, Korea)
Column temperature 40°C
UV wavelength 220 nm
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