Oryeongsan inhibits LPS-induced production of inflammatory mediators via blockade of the NF-kappaB, MAPK pathways and leads to HO-1 induction in macrophage cells

Table 3 HPLC conditions used for the analysis of OR

Item	Condition
Mobile phase	Time (min)	Water (containing 0.1% TFA)	Acetonitrile
	0	90	10
	5	90	10
	40	10	90
	40	0	100
	60	0	100
Flow rate	1.0 mL/min
Inject volume	20 μL
Column	OptimaPak C₁₈ (4.6 × 250 mm, 5 μm, RS tech Co., Daejeon, Korea)
Column temperature	40°C
UV wavelength	220 nm

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ISSN: 2662-7671